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Santa Cruz Biotechnology
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Boster Bio
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Cell Signaling Technology Inc
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Boster Bio
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Image Search Results
Journal: Journal of Alzheimer's disease : JAD
Article Title: Intraneuronal Amylin Deposition Peroxidative Membrane Injury and Increased IL-1β Synthesis in Brains of Alzheimer’s Disease Patients with Type-2 Diabetes and in Diabetic HIP Rats
doi: 10.3233/JAD-160047
Figure Lengend Snippet: Amylin-mediated peroxidative membrane injury increases IL-1β synthesis in cultured neurons. A) Lipid peroxidation measurements with C11-BODIPY581/591 in cultured primary hippocampal rat neurons incubated for two hours under control conditions (Ctl), with aggregated amylin (Amy), with 50 μM poloxamer 188 followed by aggregated amylin (S→Amy), and with 5 mM NAC for 30 min followed by aggregated amylin (NAC→Amy). B) IL-1β level, assessed by immunofluorescence, in cultured primary hippocampal rat neurons incubated under the four conditions described in panel A. Neuronal cells were identified with mouse anti-MAP2 antibody. 10 neurons/rat; n=5 rats/group. Data are presented as mean ± standard error. **p < 0.01, ***p < 0.001.
Article Snippet: Primary neuronal cells were detected with
Techniques: Membrane, Cell Culture, Incubation, Control, Immunofluorescence
Journal: Journal of neuropathology and experimental neurology
Article Title: Combining dopamine and vitamin E enhances the differentiation to cholinergic neurons of mesenchymal stem cells.
doi: 10.1093/jnen/nlaf025
Figure Lengend Snippet: Figure 3. MAP2 expression. (A) Representative images of MAP2 expression in non-induced MSCs cells, in cells treated with conventional induction media (CIM), and in cells treated with CIM supplemented with 2 concentrations of dopamine (dop) and vitamin E (Vit E). (B) The fluorescence intensity of MAP2 expression in the study groups. (P <.05 versus non- induced control, # P <.05 versus MSCs in CIM, and P <.05 versus CIM with 2.5 µM dop and 12.5 µM Vit E (n ¼ 3)).
Article Snippet: Antibodies used for Western blotting and immunostaining: Nestin (Santa Cruz Biotechnology, Dallas, TX, United States, Cat #sc-377380), TBR1 (Santa Cruz Biotechnology, Cat # sc376258), SATB2 (Santa Cruz Biotechnology, Cat #sc-81376),
Techniques: Expressing, Fluorescence, Control